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Following the simple steps Id developed, I knew Id be able to keep my blood pressure firmly under control for the rest of my life. That Id be able to be there for my children and for my family and that Id be able to truly live the life I wanted without that constant nagging fear that every day could be my last. Looking at my reams of research and scribbled notes, I knew I had something that could help thousands or maybe even millions of other people just like me. Why couldn’t this have been the Westboro Baptist Church that had been in that movie? theater? The ability to monitor and measure protein kinase activity in tumorigenesis and cancer can be indicative of and critical to the transformation process and therefore represents an attractive diagnostic strategy, with a multibillion dollar market opportunity. In this proposal, we aim to develop a novel, high-affinity fluorescent nanosensor and homogeneous assay system for monitoring the phosphorylation of a bona fide peptide substrate by c-Abl, an important kinase involved in the etiology of chronic myelogenous leukemia CML, using FRET. The technology deployed during this phase of the proposal will directly translate into the development of a sensitive platform for the diagnosis of CML. In specific Aim 1, we will synthesize the high-affinity nanosensor. In specific Aim 2, we will develop a sensitive one-step assay using this reagent. Specific Aim 3 will elaborate on this and test the effectiveness of the nanosensor in measuring c-Abl activity in CML-positive cell lysates. Given the fact that 400 disorders such as cancer have been associated with protein kinases, the development of a family of sensitive nanosensors such as the one proposed in this grant application will facilitate the diagnosis of these diseases sensitively and selectively and therefore becomes of paramount importance and will find immense utility in all the various facets of our health care, from drug discovery to patient health and point-of-care diagnostics.
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Posted by at June 28, 2015
Filed in category: Medical news,

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