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In leukocytes of patients undergoing adjuvant chemotherapy for colon cancer treatment: aidentify genes modulated by oral supplementation of zinc; b evaluate the effects of oral zinc supplementation on humoral immunity and neutrophil function. The study will be conducted on 30 adult patients aged grater than 18 years, of both genders who have undergone surgical resection of colonic neoplastic lesions without metastatic lesion. Patients will be randomized into two groups, with the first Group QT Zn, n 15 receive 70 mg/d of zinc for 16 weeks and the second will receive placebo QT Placebo Group, n 15. The study will also include 30 healthy volunteers who receive supplementation of 70 mg/d of Zn C Zn group, n 15 or placebo Group C Placebo, n 15. Zinc supplementation or placebo for all study groups will start two days before the volunteers received the pneumococcal vaccine, polyvalent 23. Fifteen days after vaccination, patients begin chemotherapy as pre-established criteria by the Oncology Service. Will be monitored the parameters of nutritional status anthropometry, bioelectrical impedance, food intake, and laboratory tests adverse effects, according to rules of the CTCAE. In the evaluation of humoral immunity, antibodies opsonization and in the pneumococcal polysaccharide will be measured. Will be evaluated the function of neutrophils by measuring DNA NETs and quantified calprotectin and elastase released in the culture supernatants of activated neutrophils. RT-qPCR will be done of genes differentially expressedDEGS on activated leukocytes. In six volunteers from each group will be analyzed global gene expression from RNA extracted from leukocytes by microarray; will be detected and correlated the molecular pathways modulated by zinc by MetaCore software GeneGo. The DEGS will be validated by RT-qPCR. Nomura has come up with its fortnight valuation monitor and finds that forward Sensex PE is at 13.3x after a minor downgrade in EPS. Earnings for Sun Pharmaceuticals, NTPC, Power Grid Corporation, GAIL and UltraTech Cement have been upgraded
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Posted by at June 28, 2015
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